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RPA is a heterotrimeric, ssDNA binding protein consisting of the 70, 32 and 14kDaa subunits.
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The svu022 with a G + C content of 73% (clustering with the gene of ATP/GTP binding protein) consists of 1239 nucleotides.
S-100 is a dimeric calcium-binding protein consisting of two subunits (α and β) [ 61].
The first gene, which is separated from P1-rr by 1,175 bp (measured from the end of the 3' P1-rr repeat to the stop codon), encodes the 4Co63-type calmodulin-binding protein consisting of 361 amino acids.
However, stimulation of mammalian TORC1 by amino acids occurs independently of Tsc1/2 and is mediated instead by a heterodimer of two small GTP-binding protein, consisting of either RagA or RagB and either RagC or RagD.
Once ssDNA is generated, it is rapidly bound by the ssDNA-binding-protein RPA, a protein consisting of three subunits: RPA1, RPA2 and RPA3.
HIF1 comprises the binding site for the heterodimeric protein consisting of HIF-1α and the constitutively expressed HIF-1β (also termed ARNT) and is a major regulator of metabolic processes as its activity is controlled by either hypoxia or oxidative stress.
We designed a fusion protein consisting of a dsRNA binding domain of transactivation response RNA binding protein (TRBP2) fused to ErbB2 binding affibody (AF) for target specific delivery of siRNA.
In the present study, a fusion protein, consisting of EGFR binding domain fused to human IL-18 mature peptide via a linker peptide of (Gly4Ser) 3, was constructed and expressed in the insect cell line Sf9 using Bac-to-Bac baculovirus expression system.
Since SBP2L possesses an RBD that differs from that in SBP2 at positions known to be required for SECIS binding, we hypothesized that a fusion protein consisting of the SBP2L SID and the SBP2 RBD may be able to support Sec incorporation in vitro.
In this type of assay, a fusion protein consisting of an RNA binding domain and GFP is tethered to an mRNA bearing the appropriate RNA binding sites [58].
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