We have generated a switchable binding protein by inserting a typical elastin turn sequence, GVPGVG, between the two IgG-binding helices of an engineered globular minidomain from Protein A. This mutant showed increased binding of IgG-molecules compared to the wild-type sequence in the presence of sodium sulphate, as measured by surface plasmon resonance spectroscopy.
A high affinity HER2 affibody [11], [12] was engineered into the C terminal of the designed Gd3+-binding protein by a flexible linker.
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As an inactive precursor, it is activated to p50, a DNA binding protein by proteasome-mediated degradation.
Like miR-214, miR-29 forms a negative feedback loop with several other critical components of the PcG complex such as Yin Yang 1 (YY1), RING, and Rybp, an YY1 binding protein, by inhibiting their expression [ 50, 51].
Analysis of macrophage pepstatin-A binding proteins by SDS-PAGE identified a primary fraction with a molecular mass of 47 52-kDa 47 52-kDaely correlathatwith the known molecloselyass of leukocorrelatedpsin-D.
Tankyrase 1 (TRF1-interacting ankyrin- related ADP-ribose polymera se 1) was originally identified as a TRF1-binding protein by using a yeast two-hybrid screen (Smith et al, 1998).
Nrf2 is not a DNA-binding protein by itself, but a strong DNA-binding activity becomes evident after the association of Nrf2 with JDP2 and/or JDP2 MafK (Supplementary Figure S4).
Meanwhile, in order to avoid the IGF-binding protein artifacts, the supernatant extracted from bone samples was pretreated (10 min) to release IGF-I from binding proteins by the use of an acidic buffer (pretreatment reagent, composition proprietary, IGF1 ELISA assay R&D Systems, Minneapolis, MN).
The most abundant unigene comprised 18 ESTs and was annotated as nucleotide binding protein, followed by a unigene comprising 12 overlapped ESTs encoding a universal stress protein.
