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Then, the binding properties were further analysed by ELISA using purified Fab fragments.
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The resulting sensorgrams showed completely overlapping binding profiles (Fig. 6A,B), which confirmed that the VEGF binding properties were unaffected by storage.
The binding properties were analyzed in competitive binding, internalization, and cell surface retention experiments.
The hypothesized binding properties were tested experimentally.
The impact of these properties was further evaluated in vivo.
However, further investigations led us to believe that binding properties are equally important.
The specific organic binding media were further analyzed by Py-GC/MS.
Residues involved in catalysis and substrate binding were further confirmed by site-directed mutagenesis experiments, and kinetic properties of RePaaH1were examined as well.
To ensure the proper spatial and temporal regulation of microtubule dynamics, the activity and binding properties of MAPs are further modulated by upstream signaling molecules.
The similarity of compound 2a to 2b and 1 with respect to structure and binding strength, differing only in the linker to the acrylamide binding group, is further evidence that that it is the covalent binding property that determines the difference in binding kinetics, rather than e.g. a slow conformational change of the target enzymes.
This relationship between the binding properties of α-ketoglutarate and its regulatory properties will be further studied in this work.
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