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To analyse their intracellular binding properties, we performed FRAP (fluorescence recovery after photobleaching) experiments.
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To assess skin mechanical properties, we performed tensile mechanical testing on excised samples.
Tests for binding properties were performed by the company DiscoverX (www.discoverx.com), using a concentration of 1 µM against ALK, MET and EGFR variants L858R and L858R_T790M) using KdELECT and scanKINETIC from DiscoverX [124, 125].
To test the binding specificities, we performed ITC to measure the equilibrium disassociation constants (Kd).
An investigation of rhesus relaxin-3 bioactivity and RXFP1 binding properties was also performed.
To demonstrate that this binding is specific, we performed a competition binding experiment.
To assess the regulatory properties of the CTCF binding site overlapping rs225014 and to test whether the rs225014 alleles directly affect the binding, we performed an EMSA.
To assess the impact of the T109M mutation on PFN1 binding to PLP, we performed molecular-dynamic simulations.
In order to further characterize the binding properties of ARE3/YY1 sequence, we performed ChIP assays in LNCap cells.
To determine whether β-casein 197 had DNA-binding capabilities, we performed a gel retardation assay.
Furthermore, DOTA-TBIA101 was nonradioactively labeled with gallium (III) trichloride to form natGa-DOTA-TBIA101 for subsequent evaluation of its bacterial binding properties and to perform in vitro cytotoxicity studies whilst preventing unnecessary radiation exposure.
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