Measurements of tandem domains did not reveal any cooperative binding properties, suggesting that the domains bind calcium independently.
This correlation underscores the significant impact that selection parameters have on resulting aptamer binding properties, suggesting that optimized in vitro selection procedures may improve resulting aptamer affinities through increased selection stringency.
Electrophoretic mobility gel shift assays and atomic force microscopy demonstrated that the protein possesses DNA-binding properties, suggesting that the transcriptional regulatory activity of SfaXII can be mediated via direct binding to DNA.
The order of their anti-proliferative activities was 6 a, d) > 5 a, d) and (5 6 a > (5 6 d, respectively, in accordance with that of their DNA binding properties, which suggested that the prolonged linker (six carbons) and piperidinyl group on the side chains are beneficial to DNA binding and the anti-tumor activity.
The fact that ChBD conserves its binding properties within BlaP suggests that its N- and C-terminal extremities are proximal in the three-dimensional structure.
DNA-binding studies suggest that DBD properties of FAX-1 and NHR-67 are at least partially evolutionarily-conserved [ 30].
Observing non-cooperative binding therefore suggests that some property of the TOG1-TOG2 linker antagonizes or counterbalances longitudinal interactions between αβ-tubulins (see below for experiments concerning the linker sequence).
Moreover, different WW domains falling in a tandem repeat manner have different binding properties to different proteins, suggesting that WW domains bind to a vast repertoire of different proteins and that they might be part of complexes bridging blocks.
A shortened IR, consisting of residues 1 601 and 650 719, displays the same insulin binding properties as the holoreceptor, suggesting that all residues needed for high affinity binding are located within these regions [80].
By SPR, uncoupled SBD (fig. 3A) and SBD-TMR (fig. 3B) gave very similar binding curves, suggesting that the presence of the fluorophore on SBD does not drastically change its binding properties.
