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The deglycosylated triple mutant was expressed and had binding properties similar to those of glycosylated FGFR4ed, but was still heterogeneous.
Thus, addition of the C-terminal domain of apoA-I to apoLp-III created a two-domain protein, with self-association, lipid and lipopolysaccharide binding properties similar to apoA-I.
Our findings identify a 159-residue RNA packaging signal that possesses dimerization and NC binding properties similar to those of the intact 5′-L and contains elements required for efficient RNA packaging.
With the advances in molecular genetics and DNA technology less immunogenic recombinant antibodies with binding properties similar to murine Abs can be designed.
The final humanized V5B2, named huScFv, contained 9 mutations in Vl and 4 mutations in Vh and had binding properties similar to mouse scFv directed against the same antigen.
Uromodulin showed binding properties similar to GP2.
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In further experiments, we established that Pat-C exhibits RNA-binding properties similar to those reported for the purified yeast Pat1-LSm1 7 complex (Chowdhury et al, 2007).
The LPL promoter has been well-characterized, and a study by Enerbäck et al. (17) has previously identified two elements in the promoter with DNA-binding properties similar to those of the forkhead family of TFs.
In addition to these major components, one of the eastern mole specimens possessed a minor cathodic Hb component that represented ~5% of the hemolysate and exhibited oxygen-binding properties similar to those of the major components (data not shown).
Here, we report that substitution of the 2′-methyl group of indomethacin with trifluoromethyl produces CF3 indomethacin, a tight-binding inhibitor with kinetic properties similar to those of indomethacin and unexpected COX-2 selectivity (IC50 mCOX-2 = 267 nM; IC50 oCOX-1 > 100 μM).
Moreover, M5 has properties similar to many other Fg-binding M proteins.
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