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Moreover, these activities are often found within modular proteins that may contain multiple domains with differing catalytic and substrate binding properties, separated by non-catalytic linker domains.
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There are generally two peaks, with field-dependent properties, separated by an excluded-volume region.
QRE is a bipartite binding site separated by less than 20 base pairs.
The beads were washed three times with 1 ml binding buffer, separated by SDS PAGE and analyzed by immunoblotting.
Despite a relatively similar molecular architecture two actin-binding sites separated by a long flexible molecular arm– and similar affinity for F-actin crosslinking/bundling proteins filamin and α-actinin modulate the mechanical properties of F-actin networks differently.
Calmodulin contains two globular EF-hand calcium-binding domains separated by a flexible linker.
Typically NFAT- and AP-1-binding sites are separated by seven or less nucleotides.
Two Cd-binding proteins were separated by DEAE-Sephadex A-25 column chromatography.
These results are generally consistent with the qualitative binding properties measured by size exclusion chromatography.
In the above examples, binding properties are regulated by an external stimulus or by allosteric control.
The bulk- and interface-related properties could be effectively separated by using impedance spectroscopy.
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