Similar assays have been previously used for the characterisation of RNA-binding properties of many RNA-binding proteins (Filipenko et al. 2004; Siomi et al. 1993).
Although several human studies have investigated the heparin-binding properties of many cytokines [ 7- 9], proteins may vary structurally between animal species and thus the effect of heparin on cytokine quantification may likewise vary between human and mouse samples.
The binding properties of biomolecules play a crucial role in many biological phenomena, especially cell adhesion.
The role of π-stacking in controlling redox and ligand binding properties of porphyrins has been of interest for many years.
Many of the binding properties of Smac can be recapitulated by a conserved tetrapeptide sequence, and small-molecule IAP antagonists that mimic the action of Smac have been designed to potentiate apoptosis in cancer cells [ 29– 329.
Theoretical calculations of electrostatic interactions can account quantitatively for many of the observed binding properties of peripheral membrane proteins.
A calibration of the ratio for [Ca2+]i was not performed in view of the many uncertainties related to the binding properties of fura 2 with Ca2+ inside of smooth muscle cells.
Often the dyes being attached are hydrophobic and if too many are attached it is reasonable that the binding properties of the protein could be altered.
Many studies have generated convincing evidence of the unique antigenic and binding properties of P. falciparum iEs collected from the placenta.
