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Surface display of metal-binding peptides, or protein domains into which metal binding properties has been engineered by combinatorial protein engineering, have been exploited to create staphylococcal bioadsorbents for potential environmental or biosensor applications.
The impact of this modification on the protein's structure and dynamics in free solution and on its membrane binding properties has been evaluated by high-resolution nuclear magnetic resonance and circular dichroism (CD) spectroscopy.
Such a hybrid VL domain with full binding properties has been previously realized by grafting λ CDRs on a κ framework [ 11], and we have also successfully grafted the CDRs from a mouse κ scFv directed against the human papilloma virus E6 protein [ 44] on the scFv13R4 λ framework (manuscript in preparation).
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Although the catalytic function of the exosome core has been lost during evolution, the substrate recruitment and binding properties have been conserved from prokaryotes to eukaryotes.
Indeed, adaptative mechanisms including modified DAT binding properties have been shown following partial nigrostriatal lesions [32].
PfEMP1 variants mediate adhesion of IEs to different host endothelial receptors, and different binding properties have been associated with distinct patterns of sequestration and pathogenesis [8].
Their versatile binding properties have been structurally and functionally studied since the early 90's providing a wealth of data on how antibodies in principle recognize and bind their target molecules [26], [27].
Its receptor binding properties have been reported previously and the highest affinity receptors are FGFR2-IIIc, FGFR3-IIIc and FGFR4 (Ornitz et al, 1996).
As a result, similar ERE binding properties have been associated with a partially distinct spectrum of ligands for each receptor, although with similar affinities for estrogen.
Understanding the role of glycosylation on HA on its glycan receptor-binding properties has been an active area of research.
In order to explore the relationship between the structure and biological properties, the DNA-binding properties have been investigated by electronic absorption, fluorescence, and viscosity measurements.
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