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Indirect Binding Assay: The aluminum binding propensity of each sorting round, a population consisting of a single isolate or the empty display vector (negative control) were compared by quantifying the number of cells recovered from the aluminum surface.
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The intrinsic binding constant, Kb values of 1 and 3, were calculated and found to be 2.76 × 104 and 3.84 × 104 M−1, respectively which implicates greater binding propensity of diphenyltin analog 3. Furthermore, a mechanistic insight with 5′-GMP was envisaged for 1 and 3.
Interestingly, we noticed few differences in the binding propensity of residues among different organisms.
We have evaluated the influence of RNA base sequence on the binding propensity of amino acid residues in nucleotides.
Authors' response: We have evaluated the influence of RNA base sequence on binding propensity of amino acid residues in nucleotides.
The catRAPID algorithm exploits predictions of secondary structure, hydrogen bonding and van der Waals' contributions to estimate the binding propensity of protein and RNA molecules.
These results are similar to the binding propensity of residues obtained with energy based approach in a set of 81protein-RNAprotein-RNA [ 4].
We have computed the normalized binding propensity of nucleotides in E. coli, H. sapiens, S. cerevisiae, thermophiles and archaea, and the results are presented in Figure 2.
The maximum discriminant function score across all windows in a protein can be used as the CaM binding propensity of that protein.
To further evaluate the DNA binding propensity of these mutant proteins, we assayed HSF1 DNA binding at the IL-6 promoter where HSF1 has previously been shown to bind constitutively, even in the absence of stress (Takii et al. 2010).
We observed that the residues Ala, Val, Leu, Ile, Asp and Glu with the majority of hydrophobic residues have the normalized binding propensity of less than 2 and hence are not preferred at the binding sites.
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