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H-NS and Hha binding profiles were determined using a slight modification of the previously described ChIP-chip and ChAP-chip methods.
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Baseline biochemical parameters, including hemoglobin, total iron-binding capacity (TIBC), creatinine, calcium, phosphate, albumin, C-reactive protein (CRP), and lipid profiles, were determined before the dialysis session.
Charge and mass profiles were determined.
Lipid profiles were determined by F.P.L.C. (Dionex).
Cell cycle profiles were determined by flow cytometry (Fig. 4I).
The expression profiles were determined using miRCURY™ LNA miRNA Arrays.
Inflammation markers and lipid profile were determined by standard methods.
Based on the crystal structure of ADT in complex with an oligopeptide segment corresponding to the core binding site of LHB, a sequence-specific amino acid preference profile was determined systematically for the ADT-binding peptides using structural bioinformatics approach.
The transcriptional profile was determined using Affymetrix U133plus2 microarrays.
Porphyrin profile was determined by HPLC.
% overlaps of the motifs with p63 binding profile were also determined.
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