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Furlong and colleagues have previously determined the genome-wide binding profiles of a set of key Drosophila mesodermal regulatory TFs, including Tin, Twi and Mef2 (Zinzen et al., 2009).
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We established genome-wide binding profiles of all male-specific Fru isoforms throughout development in the nervous system using a DNA adenine methyltransferase identification (DamID) approach [ 11].
The resulting binding profiles of all five insulator proteins are generally characterized by sharp peaks of local enrichment (Fig. 2a, Fig. S2a).
Unlike IPOD, GeF-seq can visualize the binding profile of a specific target protein at a resolution seen at the in vitro footprinting level.
These methods fall short when used to characterize the binding profile of a novel active site or one for which a template is not available.
Both, the peptides and PAs offered a expected binding profile of a SPR measurement with the association process, where the peptides and micelles interact with the immobilised hemagglutinin.
We note that the binding profile of a TF can also be calculated based on the ChIP-chip data of the TF in the same way.
Instead, it is important to study the possible binding profile of a given drug to all those proteins to which it is likely to be exposed.
A colorimetric on-bead western assay was used to evaluate the binding profile of a range of GST-tagged domains to the H3 library (residues 1 10) in an unbiased manner.
It is conceivable that the more selective ligand-binding profiles of a viral Bcl-2 protein reflect the circumstance that only specific pro-apoptotic Bcl-2 proteins require targeting during viral infection.
The comparison of genome-wide binding profiles of Tip60 and c-Myc, a somatic cell reprogramming factor that binds predominantly to active genes in mESCs, demonstrate that Tip60 and c-Myc co-bind at 50 60 % of their binding sites.
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