For each of the three proteins, we obtained the intensity values [binding ratio (log2) and P value] of more than 40000 probes that covered the entire yeast genome and constructed binding profiles for three proteins (Supplementary Figure S1).
We generated genome-wide binding profiles for three Hox proteins, Ubx, Abd-A and Abd-B, following transient expression in Drosophila Kc167 cells, revealing clear target specificity and a striking influence of chromatin accessibility.
We conclude that we have successfully generated high-resolution genome-wide binding profiles for five insulator proteins, together with the NL interaction profile, in one and the same Drosophila cell type.
A comprehensive study compared ligand-binding profiles for eight human SULTs (87); out of SULT1C-1 to -3, SULT1B1, SULT1A1, SULT1A3, SULT2A1, and SULT1E1, E1 only bound to SULT1E1; 2-hydroxyestradiol only bound to SULT1C3, 4A1, 2A1, and 1E1; DHEAS only bound to SULT2A1 and 1E1; and the bile acid lithocholic acid only bound to SULT2A1 and 1E1.
The comparison of AChE-lectin binding profiles for the two mannose specific lectins, LCA and Con A, give no definitive conclusion because the enzyme in plasma does not reproduce the binding pattern of a single tissue, probably due to the diverse number of cellular origins.
A similar observation is made for data sets obtained with the other two antibodies against the unmodified CTD and the Ser5-P; however, the switch between early and late binding profiles for these two forms occurs earlier between T3 (24 hpi) and T4 (32 hpi).
In this study, we took advantage of a lectin microarray with 91 lectins that we have constructed previously [ 20], generated the lectin binding profiles for sperm of five mammals-human, boar, rabbit, bull and goat.
Julia Zeitlinger, Alexander Stark and colleagues report genome-wide binding profiles for the transcription factor Twist across six Drosophila species.
(b) ChIP-seq binding profiles for Med1, Smad3 and PU.1 at the locus of Foxo1 gene.
With these microarrays, we individually and competitively determined the binding profiles of five gp120 binding proteins, established the carbohydrate structural requirements for these interactions, and identified a potential strategy for HIV vaccine development.
We have presented a method for predicting PDZ domain binding specificity, used in the DREAM4 peptide recognition domain challenge to determine ligand binding profiles of five multi-mutant ERBB2IP-1 Pdomainsins.
