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ATP agarose was used in a binding procedure as previously described with minor modifications (28, 29, 32).
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Analyzed genomes were scanned with the constructed Zur-binding motif profile using the Genome Explorer software [ 68], and the identified genes with candidate Zur-binding sites were analyzed by the consistency check comparative procedure as previously described [ 6].
We followed the same model selection procedure as previously described.
using standard neurosurgical procedures, as previously described [51].
cDNA was synthesized using standard procedures as previously described [ 24].
The parabiosis was performed following procedures as previously described [ 31].
The procedures were performed essentially as previously described [63], [64].
O157LPS binding was detected as previously described [21].
3H-CGP12177 whole cell binding was performed as previously described [25].
ATP-γ35S binding was quantified as previously described [94].
The transformation procedure was as previously described [ 9, 40- 42].
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