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The rank order of preferred DNA sequences also differs, further demonstrating distinct DNA binding preferences.
During hyphal induction, however, when Tpk2 binds predominantly within promoter regions, a completely different set of binding preferences was found.
Solomon, D.L., Amati, B. & Land, H. Distinct DNA binding preferences for the c-Myc/Max and Max/Max dimers.
Although useful for mapping regulatory genomic regions, none of these datasets provide direct access to allelic binding preferences.
Here we report that the fungal SREBPs diversified their DNA binding preferences concomitantly with an expansion in function.
Cutting-edge technologies have identified genomic DNA regulatory elements and revealed the binding preferences for many transcription factors.
Scientists leverage one step, unbiased method to characterize the binding preferences of more than 70 human RNA-binding proteins.
In this study, we show that nonconsensus protein-DNA binding significantly influences PIC binding preferences.
Martha Bulyk has taken the gene chip technology originally developed to measure gene activity and adapted it to determine the DNA binding preferences of proteins.
Consistent with these binding preferences, EphB4 markedly disrupted the ephrinB2/TBC1d24 interaction in Co-IPs (Fig. 6b), and EphB2 had little effect (Fig. 6b).
Thus, our results illustrate how diversification in DNA binding preferences enabled the functional expansion of a family of eukaryotic transcription regulators.
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