Additional file 1: PDE10A binding potential and specific activity.
The genes that are bound and/or regulated under these conditions are assessed computationally in terms of Leu3 binding potential, the evolutionary conservation of that binding potential, and the enrichment of functionally related genes.
In addition to this extended binding potential, global binding data of PML RAR α using ChIP-seq revealed PML RAR α binding to the RAR α, RAR β and RAR γ genes itself (Table 1), suggesting that expression of these proteins is directly regulated by PML RAR α (Martens et al, 2010).
Our approach scores human and macaque sequence variants in an MAF block for TF-binding potential and compares predicted binding sites between the two species (Kostka D, Holloway AK, Pollard KS, manuscript in preparation).
This will be particularly useful for studying binding of agents to receptors, allowing values to be derived for receptor number, binding affinity and binding potential.
The energy based methods build on the approximation of binding potentials or binding energies [16].
Independently evolved binding sites can contribute to the binding potential score, and to the conservation of this score.
We obtained arterial blood samples for kinetic data analysis and found that the compound had a differential distribution in brain regions, with the highest binding potentials in the frontal and temporal cortices, intermediate binding potential in the thalamus, and low binding potentials in the striatum, hypothalamus, and brainstem.
For example, Kaye et al. (2009) reported elevated 5-HT metabolite levels, as well as elevated binding potential for postsynaptic 5-HT1a receptors and diminished binding potential for 5-HT2a receptors in recovered AN patients [ 53- 55].
As expected, genes whose promoters are bound according to the ChIP experiment are, as a group, enriched in Leu3 binding potential, demonstrating a correlation between predicted binding and observed binding (Fig 1B).
