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Exact(8)
To test this possibility we compared VNTR domain sizes with DC-SIGN binding potency of the corresponding milks.
To quantitatively observe the change with different doses, we conducted a dose response analysis to investigate the binding potency of the MIX, bioactivated MIX, and SRM 2585.
Overall, we conclude that the FP assay was appropriate and efficient to evaluate the binding potency of the tested compounds and dust extracts.
In addition, the binding potency of the house dust in our study might be underestimated due to FP interference from the dust matrix.
It might be possible that compounds in dust, such as TBB, TBPH, PBDEs, and DEHP, could be metabolized to PPARγ-active ligands after incubation, which was supported by the increased binding potency of the prepared MIX containing these chemicals.
Briefly, we used a commercially available high-throughput ligand binding assay (PolarScreen™ PPARγ-Competitor Assay Kit; Invitrogen, Carlsbad, CA) to investigate the binding potency of the tested compounds to PPARγ LBD.
Similar(52)
The binding potency of those dust extracts was only slightly lower than the positive control (12.5 μM of rosiglitazone), which could completely inhibit the binding between the PPARγ LBD and PPARγ Green probe.
The in vitro binding potency of these new analogs toward S1PR1 was determined.
For comparison of the binding potencies of the dust extracts, all FP values of the dust samples were normalized to the procedural blank.
The substitution of the B8 site with d-ProB8 and NMeAlaB8 amino acids (adopting mostly dihedral angles from the right half of the Ramachandran plot) also had a deleterious effect on the binding potencies of the resulting analogues.
They constituted less than 1%% of the TTR-binding potency of the unfractionated extract.
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