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We also found that binding positions of the receptor analogs relative to HAs are highly conserved and the backbone conformation at receptor binding region is almost fixed, when analyzing the co-crystal structures involving HAs from different type A viruses.
The ZDOCK algorithm in Discovery Studio uses the fast Fourier transform (FFT) correlation techniques and searches all possible binding positions of the two proteins.
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In designing the synthetic derivatives, we focused on the binding position of the fatty chain.
The simulations reveal a strong dependence of the conformational ensembles on type and binding position of the bound substrates and a nonsymmetric behavior of the lid domains.
This study clearly shows that the number and the binding position of the fatty chain are critical for protective activity against ER stress-dependent cell death.
The correct binding position of the ligand was obtained after the MDF stage of Shaker with an improvement of RMSD from 6.8 to 1.7 Å (Fig. 7, Additional file 3: Supporting Movie 2).
It has been reported that the binding position of the antisense RNA may determine gene-silencing mechanisms [34], [35].
Second, the HA structure was aligned against known HA-receptor complex templates and the binding position of the receptor analog in the HA receptor binding domain was initialized.
Individual primers were named as follows: 'EHDV' representing Epizootic haemorrhagic disease virus, followed by a number indicating the serotype; 'S2' to indicate they target Seg-2; 'F' representing forward, or 'R' representing reverse primers; and a number corresponding to the nucleotide binding position of the primer in Seg-2.
The third one is using ChIP-chip (Chromatin ImmunoPrecipitation followed by microarray analysis) to profile the binding position of the RNA polymerase II preinitiation complex [ 31].
If such a 3-dimensional structure is available, PepSite analyses the SLiM-binding domain for the likely binding position of the peptide, producing a putative binary complex and a score for the likelihood of the interaction.
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