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Because the β-clamp is a dimer, there are two polymerase binding pockets present on a single clamp.
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ATP binding at the NBD opens up the substrate binding pocket present in substrate binding domain.
The structure activity relationship is less well defined, as no distinct binding pockets are present.
It has several binding sites on each fibrinogen-like domain: an outer binding pocket, also present in H-and M-ficolins, together with three unique sites, which form a continuous binding surface for various acetylated and neutral carbohydrate ligands.
By "biological affinity," binding of ligands or proteins to specific binding sites or pockets present in target proteins (e.g., receptors or enzymes) is meant.
Here, we present two new algorithms for designing tailored ligand binding pockets on the protein surface that account for backbone and side chain flexibility.
Molecular docking studies employing the reported crystal structure revealed five possible binding pockets on mitoNEET.
Two binding pockets on the ΔF508-NBD1 ΔF508-NBD1
Of the 10 minimotif groups, many used different binding pockets on the SH3 domain.
This finding was supported by analysis of the binding pocket consensus of the promiscuous PDZ cluster, which revealed the preference of a histidine residue at position 14 (αB1, present in 80% of the binding pockets) and a valine at position 16 (αB5, present in 70% of the binding pockets; Fig. 4C panel b).
The binding pocket of KQTSV on the PDZ domain is presented with grey surface.
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