Our discovered stable secondary structures around the promoter regions may contribute to the interaction between splicing and transcription via serving as stable binding platforms of the transcription/splicing regulators.
The highly variable complementarity-determining regions of TCR on the surface of CD8+ cytotoxic T lymphocytes (CTL) interact with both the peptide and the peptide-binding platform of the MHCI molecule to confer peptide-MHCI (pMHCI) specificity 2. Almost all nucleated cells express MHCI, thereby enabling CTL to scan for internal anomalies.
Multimeric forms of DT227/8KA HLA-A68, WT HLA-A68 and CD8-enhanced (V245A) HLA-A68 antigens all bind to cell surface TCR equally well, suggesting that these substitutions do not interfere with the α1/α2 peptide-binding platform of the HLA-A68 molecule contacted by the TCR (13 and data not shown).
Therefore, TRAF2 is likely to function as a binding platform that facilitates dimerisation of the RING domains of cIAPs.
Sin3 associates with RBP2 (retinol-binding protein 2) through SAP30 (Sin3-associated protein 30) and recruits histone methyltransferase Suv39H1 (suppressor of variegation 3 9 homologue 1) that results in trimethylation of hypoacetylated H3K9 and provides a binding platform for the chromodomain of HP1 (heterochromatin-binding protein 1) protein [ 10, 37, 53, 54].
Microtubule plus ends are dynamic binding platforms for a variety of proteins, collectively referred to as +TIPs (plus-end tracking proteins) (Akhmanova and Steinmetz, 2008).
Histone acetylation may also facilitate DNA double strand break (DSB) repair by creating a binding platform to promote recruitment of remodeling complexes at the sites of DSBs [reviewed in (33)].
These findings, along with a comparison of the structural data, indicate that loop 2 configures a binding platform for the acceptance of the incoming primer strand, which is remodeled to the "open" conformation during the assembly of the preternary complex to direct the arrival of the primer terminus into the active site.
It can either constitute a binding platform for the regulators of transcriptional machinery or temporarily interfere with the binding or activity of factors targeted to adjacent residues.
This binding mode explains the large number of EB binding sites at growing microtubule ends (Maurer et al., 2011) and emphasizes that the microtubule-end region can serve as a binding platform for many hundreds of +TIP molecules.
