Sentence examples for binding plates were incubated from inspiring English sources

96 well ELISA high binding plates were incubated overnight at 4°C with ephrinA1-Fc 1 µg/cm2, 100 µl per well.

High-binding plates were incubated with dilutions of TGF-β in phosphate buffer overnight, and subsequently blocked, incubated with primary antibodies against TGF-β, biotinylated secondary antibodies, and a GOx-avidin conjugate at 100 µg/mL.

After adding the radioligands and the blocking for nonspecific binding, the plates were incubated for 1 h at 4 °C.

In the binding assay, the plates were incubated with the various dilutions of samples at 37°C for 2 h followed by washing three times with PBST.

After two washes with binding buffer, the plates were incubated with I-RGDyK (2 nmol/L) in the presence of various concentrations of ligand ranging from 1 pM to 10 μM for 16 h at room temperature, followed by three washes with binding buffer.

In 96-well plates, serial dilution of calycosin (1 nM to 3×105 nM) or the ER agonist 17-β-estradiol (104 nM to 10−2 nM) were added to ER/Fluormone™ ES2 complexes to compete with ES2 for binding to ER. Plates were incubated at room temperature for 2 h and fluorescent polarization values measured using a Multilabel Counter (Perkin Elmer, Singapore).

The medium was then removed from the cells and 100 mL of 1X dye binding solution was added and plates were incubated for one hour at 37°C.

To analyze the ability of PLG to inhibit the binding of CFH to HcpA, plates were incubated simultaneously with constant amounts of CFH (3 µg/ml) and different amounts of PLG (0.001 100 µg/ml).

The plates were incubated at 4°C overnight to allow binding.

Subsequently plates were incubated for 1 h at 37° and Ab binding to gp120 was determined.

Plates were incubated on ice for 10 minutes to allow binding of particles.