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To prevent non-specific binding, plates were blocked with 1.5%BSA/TBS/0.11% Tween.
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To avoid non-specific binding, the plates were blocked with 1% bovine serum albumin (BSA) in PBS.
Nonspecific binding sites on microtiter plates were blocked with 2% BSA in PBS and incubated for 90 minutes at 37°C.
The coating solution was aspirated off, and unoccupied binding sites on the plates were blocked with 2%% bovine serum albumin in PBS at 37 °C for 1 h.
After washing for three times with the binding buffer, the incubated plates were blocked with 3% (w/v) BSA solution for 2 hours at 37°C, and unbound BSA was removed.
After washing for three times with binding buffer, the incubated plates were blocked with 3% (w/v) BSA solution for 2 hours at 37°C and again washed three times in order to completely remove unbound BSA.
After washing for three times with binding buffer, the incubated plates were blocked with 3% (w/v) BSA solution for 2 hours at 37°C and then again washed three times in order to completely remove unbound BSA.
The non-binding sites of the ELISA plates were blocked with 200 μl/well of 2% bovine serum albumin in PBS for 2 hours at room temperature.
The plates were blocked with 1% BSA in PBS at room temperature for 3 h to prevent nonspecific binding.
Plates were blocked with 0.1% BSA.
Plates were blocked with PBS, 1% bovine serum albumin.
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