Sentence examples for binding phage from the from inspiring English sources

These PLAP-conjugated beads were used for selection of PLAP binding phage from the synthetic immunoglobulin library.

To elute the bound phage using competitive elution, 100 μL of parathion-methyl (100 ng mL 1 in PBS) was added to each well with shaking for 1 h to compete with the binding phage from the coating antibody.

Then, the plate was washed eight times with PBST, and 100 μL of analyte was added for 1 h at 37 °C to compete with the binding phage from the coating antibody.

After incubating for 1 h at 37 °C, the plate was washed eight times with PBST and 100 μL of parathion-methyl (100 ng mL 1 in PBS) was added for 1 h at 37 °C to compete with the binding phage from the coating antibody.

To separate phages binding to the ligand from non-binders, the ligand is immobilized on a solid carrier allowing for easy separation of the liquid phase, which contains predominantly non-binding phages, from the solid phase, which contains mainly binding phages.

To investigate whether the critical Tyr3, Tyr6, and Trp10 can be replaced by other amino acids, we isolated the EphA4-binding phage from libraries with variable residues at these positions.

To prevent the selection of streptavidin-binding clones, phages from the library were pre-incubated in wells coated with streptavidin (1 2×10 phages/well) and then transferred in the selection plate for 1 h at 20°C.

However, an increase in the eluted phages of approximately 10 times between the first and second panning is occasionally also seen in experiments from which no true binding phage can be identified.

The binding specificity of these anti-V3 scFvs was checked with phage from the 24 well plate and PEG precipitated purified phage.

The result showed that the subtraction is rather effective to exclude non-specific binding phages.

Selected phage pools also showed increased binding efficiency to ES cells, from 8×10−8 to 1×10−4, indicating successful accumulation of specific ES cell binding phages (Figure 2B).