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Our engineered collagen binding phage could be a useful platform for abnormal collagen imaging and drug delivery in various collagen-related diseases.
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Additionally, the combination of two different binding motifs into a single bifunctional phage could be useful for the generation of new composite materials.
Similar to the concept of a mixed viral vaccine, phages also could be used as a portion of a cocktail for broad host range lysis and more phage could be added into suit the resistance situation (Chan et al. 2013).
Hence, chitosan film containing liposome-encapsulated phage could be a promising antibacterial packaging for beef preservation.
Unbound phage could be washed away, leaving bound phage, a proportion of which was specific for the target antigen.
A large panel of serotype specific phage expressing botulinum binding scFv could be selected from both libraries.
Since the phages showed specific binding on C. albicans cell surface we tested if the specificity of binding could be attributed to carbohydrate or proteinacious moieties on the cell surface.
This binding could be inhibited by heparin.
Phage types could be further divided by PFGE.
The phage plaques could be seen visible after 6-8 h.
With an estimated 10 phages worldwide, phage-encoded endolysins could be a vast resource for exploiting antibacterial agents against pathogens.
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