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Taken together, we showed that murine TAP is more skewed than human TAP towards binding peptides based on their C-terminus alone.
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Thus, using multiple peptide templates and selecting the binding peptide based on the lowest energy criteria would be the best approach unless there is some prior information about the best template peptide.
We found differences statistically significant on the binding affinity of peptides based on their content of cysteine, hydrophobic and polar amino acids, mainly containing alcohols.
This binding was inhibited by peptides based on sequences unique to PKC betaII; thus defining an actin-binding site in PKC betaII that is not present in PKC betaI.
This phosphorylation resulted in reduced binding of proline-rich peptides based on the structure of the Sos exchange protein [ 29].
Mcl-1 bound with high affinity to VDAC1 and 3 isoforms but only very weakly to VDAC2 and binding was disrupted by peptides based on the VDAC1 sequence.
Phage display technology has been used for exploring novel peptides acting on GPCRs, but it is unclear whether the identified peptides functionally modulate targets because the technology selects peptides based on binding ability but not functional activity to targets.
A truncated variant of BamD was used to demonstrate binding of this lipoprotein to synthetic peptides based on the OMP C-terminal targeting sequence, and this interaction occurred at the same binding pocket as the interaction with BamC [116,125].
Our objective was to develop small peptides based on essential binding elements of the natural protein inhibitor, Tendamistat.
Sorting these peptides based on their helicity and binding activity, the authors concluded that the binding affinity increases with increase in the helical content.
Similarly, short α-helical peptides, based on the heme binding peptide fragment of myoglobin, have been covalently attached to deuterohem by a similar amide-bond attachment strategy, yielding compounds known as mimochromes.
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