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GATA1 binding peaks were obtained from [ 15].
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Additionally, peak height was adopted instead of peak area because sharp peaks were obtained.
After chromatography, two major peaks were obtained and they were designated as peak-I and peak-II (Figure 2).
As shown in FIG. 2 three important peaks were obtained.
Two distinct component peaks were obtained from the bispecific serum.
The ratio for each peak is obtained.
A single peak was obtained for all proteins and fractions within this peak pooled.
The binding peaks were determined by the peak finding algorithm provided in the TiMAT package.
This analysis showed that 23.8% of all FXR binding peaks were located close to LRH-1 peaks.
The binding mechanism, binding constants, binding sites and binding distance were obtained.
Peak parameters (peak area, height, width, as well as peak spectrum) were obtained using the instrument software.
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