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Potential SRC-1 binding peaks were identified using three different algorithms: MACS 1.4.1 [ 9], BayesPeak[ 10], and T-PIC [ 8].
The target genes corresponding to the TF binding peaks were identified according to PAVIS criteria by using our own scripts [ 32].
ARF6 binding peaks were identified using ChIP-Seq analysis R (CSAR) software with parameters (backg = 10, norm = −1, test = 'Ratio', times = 1e6, digits = 2) (Muino et al., 2011).
DNA binding peaks were identified by using the statistical model and methodology described at (http://chipanalysis.genomecenter.ucdavis.edu/cgi-bin/tamalpais.cgi) (Bieda et al. 2006) using stringent parameters for peak identification (98th percentile threshold and p < 0.0001).
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ATF4-binding
In addition, a significant binding peak was identified at SIRT4 gene promoter in CtBP genome-wide-binding profile analysis (Supplementary Figure 3A).
A high confidence MYCN-binding peak was identified in the LFG proximal promoter that disappeared when MYCN expression was repressed by doxycycline treatment.
Overrepresented TF binding sites within peaks were identified using the RegionMiner tool.
A total of thirty six such peaks were identified and Table 3 lists the genes located immediately downstream of the binding sites.
A total of 185 peaks were identified.
Two clear peaks were identified in Col2a1.
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