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For DWG, BEAF-32, CTCF and GAF respectively 4.6%, 14.3%, 17.5% and 33.7% of the binding peaks are located within LADs, while 40.6% is expected by chance.
Thus we have included a considerable portion of CDRs in the datasets, because some binding peaks are located in CDRs.
We also found that some portions (18% for ChIP-seq, 17% for ChIP-PET, 11% for ChIP-chip) of Pol-II binding peaks are located in the 3' ends (including 3' Core, 3' Proximal and 3' Distal regions).
We also found that a relatively small number of ERα binding peaks are located in a known 5'TSS region (9% for ChIP-seq, 6% for ChIP-PET, 6% for ChIP-chip).
A big portion of ERα binding peaks are located within intra-genic regions (38% for ChIP-seq, 37% for ChIP-PET, 58% for ChIP-chip) as well as gene desert regions (14% for ChIP-seq, 20% for ChIP-PET, 12% for ChIP-chip), 100 kb far away from known 5'TSSs and 3'TSSs (Additional file 2, Figure S1B).
Our results showed that 18% for ChIP-seq, 44% for ChIP-PET, 45% for ChIP-chip of Pol-II binding peaks are located within Promoter regions (defined as 2 kb upstream to 2 kb downstream, including 5' Core, 5'TSS and WithinGene Core regions) of a known transcription start site (5'TSS) (Additional file 1, Table S1 and Additional file 2, Figure S1).
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This analysis showed that 23.8% of all FXR binding peaks were located close to LRH-1 peaks.
When we evaluated where the LRH-1 binding peaks were located with respect to mRNA encoding genes, we were surprised to find that LRH-1 binding sites were predominantly located in the promoter regions (2 kb 5', 24.1%), and 5'UTR (22%) relative to the transcription start site (TSS) for known genes.
As known, the peaks are located at binding energy of about 899, 903, and 916 eV normally used as the spectroscopic marker to detect the presence of Ce4+ state.
The binding energies of Fe 2p3/2 and 2p1/2 peaks are located at 707.8 and 721.3 eV, respectively, confirming the presence of Fe3+ state in Fe2O3 (Fig. S5b).
Most of the major peaks are located in this zone.
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