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Moreover, the binding pattern was not affected by the addition of purified IgG, used as a negative control (Fig. 5D, lane 6).
In contrast, the binding pattern was not affected by the addition of the antibodies directed against other Sp proteins (Sp2 and Sp4) (data not shown), showing that the two complexes did not seem to involve these other proteins.
Similar(55)
On the whole, no modification of the CycG binding pattern was observed (data not shown) suggesting that CycG recruitment does not depend on Corto.
A similar allele-specific binding pattern was obtained using nuclear extract derived from human THP-1 cells (data not shown).
Similar binding pattern was observed with ETS-1 oligo and nuclear extract from mouse αTN4-1 cells (data not shown).
Similar binding pattern was observed with SP-1/SP-3 oligo and nuclear extract from mouse αTN4-1 cells (data not shown).
Similar binding pattern was observed with AP-2α oligo and nuclear extract from mouse αTN4-1 cells (data not shown).
The same binding pattern was observed for U36 as expected.
This pattern is not perfect.
This pattern isn't random.
While data redundancy may exist as shown in Table 2, it is possible that some binding site sequence patterns are not exhaustive.
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