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Co-immunoprecipitation has been widely used to identify protein binding partners within a native biological tissue.
The dataset is noisy as it includes results from multiple laboratories and varying methodologies, some of which might not have been sensitive enough to identify direct binding partners within a collection (complex, aggregate) of proteins.
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Secondly, A-type lamins interact with a number of binding partners within the nucleus, which in turn interact with and influence the activity of important growth regulators.
It is now possible for a researcher to take a model of their protein of interest that has been energy minimised, and evaluate its binding partners within days compared to weeks/months using a single processor.
In fact, the Protein Binding function of GATHER identified a significant enrichment of STAT3 binding partners within the upregulated set of genes and GSEA analysis reported significant overlap between the upregulated genes and gene sets related to IL-6 (interleukin 6), which is an activator of STAT3.
The regulation of localization of FAK and Pyk2 to focal adhesions can be independently influenced by the presence or absence of specific binding partners, for example, gelsolin has been reported to bind Pyk2 but not FAK [40] as well as differences in the affinity of FAK and Pyk2 for shared binding partners within the focal adhesions, such as paxillin [41] [43].
We first screened for Id1 binding partners within our ES-cell cDNA library.
This recruits several other proteins that have binding partners within the synaptophysin/cholesterol environment, including, when available, synaptobrevin or synaptotagmin.
Yap and Taz have multiple binding partners within the cytoplasm and nucleus, the best-characterized of which are the Tead transcription factors.
Whether or not free β-catenin was higher in the nucleus or cytosol at steady state, depends on the active transporters and the affinity of the binding partners within each compartment.
The three essential components, AcrA, AcrB, and TolC, must function in concert with each respective binding partner within the complex.
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