Sentence examples for binding partners were eluted from inspiring English sources

p120 binding partners were eluted by incubating the beads with RIPA buffer supplemented with 50 mM DTT in for 30 minutes at 37°C with end-over-end rotation.

A major difference between ReCLIP and other methods is that only putative binding partners are eluted when the crosslinks are cleaved (see Figure 3a).

The immunoprecipitated protein and any potential binding partners are eluted by boiling the matrix in SDS-containing buffer, which disrupts the protein A-immunoglobulin (Ig) interaction [1].

In our model, p120 was directly immunoprecipitated under stringent conditions and binding partners were selectively eluted from the p120 "bait" by chemical cleavage of the crosslinker.

Another typical case is that binding partners are phosphorylated on binding sites and bind other effectors.

It is clear that the RNA levels of some binding partners are increased and of some binding partners decreased.

For example, HSP30, encoding a Nha1 binding partner, and ARR3, encoding an Ena2 binding partners are strongly up-regulated.

The sample was washed and the bead-binding complexes were eluted with elution buffer [ 48].

After washing with binding buffer (PIERCE), the binding proteins were eluted with 0.1 M glycine-HCl (pH 2.2).

The specific Tpm1 binding proteins were eluted using different buffers: 0.5 M KCl or 3 M urea in HEK buffer.

Binding proteins were eluted with a salt gradient of 0 - 1 M NaCl.