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Neither of these binding partners were detected in parallel purifications from an untagged negative control strain or from several hundred other TAP-tagged bait proteins [27].
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Neither of these binding partners was detected in parallel purifications from an untagged negative control strain or from several hundred previously studied SPA-tagged bait proteins [13].
Interaction partners were detected by using streptavidin-HRP and ECL.
In addition to KAP3, KIF3A and KIF3B, which are KAP3-binding partners, were weakly detected around fraction no.
A complete list of binding partners is shown in Figure 5A and the number of peptides detected in each biological sample is shown in Additional file 9: Table S4.
Another typical case is that binding partners are phosphorylated on binding sites and bind other effectors.
It is clear that the RNA levels of some binding partners are increased and of some binding partners decreased.
For example, HSP30, encoding a Nha1 binding partner, and ARR3, encoding an Ena2 binding partners are strongly up-regulated.
With the iodinated TxR agonist [125I]BOP, TxA2 binding sites were detected throughout the kidney.
30 PLAP binding clones were detected.
Highest lipid-binding affinities were detected for SUMO-2 interaction with PI3P and PI 3,4,5 P3, indicating that both binding partners could serve as potential pathways to the ESCRT complex.
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