In a hunt for new TIMAP-binding partners, we found that RACK1 (receptor of activated kinase 1, a.k.a. guanine nucleotide-binding protein subunit beta-2-like 1, GNB2L1, or human lung cancer oncogene 7 protein) interacts with TIMAP in pulmonary artery endothelial cells.
The new HGF binding partner we find on the cell surface may help to clarify conflicts in the past literature about Met expression and HGF response in cancer cells.
Of these 24 partners we found 7 RNA-binding proteins, 5 mRNA translation regulators, 4 DNA-binding proteins involved in transcriptional control, 4 proteins involved in microtubule assembly, and 4 interactors either with an unknown function or involved in cell signaling and mitochondrial regulatory activity.
Very recently, the discoverers of TPLATE used tandem affinity purification to identify TPLATE binding partners, and found the Arabidopsis orthologues of the TSET components that we identified independently in the present study (Gadeyne et al., 2014).
Among the binding partners found was a diaphanous homolog (SmDia), which was characterized further.
A multitude of Map3k1 binding partners have been found to date by a wide variety of molecular approaches (Table 1).
Recently both Lpd and its binding partners Ena/VASP were found to interact with the Scar/WAVE regulatory complex (Law et al., 2013; Chen et al., 2014), an important activator of the Arp2/3 complex at the leading edge.
A previous analysis of adhesion to bilayers containing the natural CD2 binding
Searching for FBPase binding partners in the nucleus, we found that the enzyme could interact with several proteins participating in the formation of interchromatin granule clusters (IGC), the structures involved in mRNA processing (Saitoh et al. 2004).
However, it is unclear how binding partners find each other in the context of the crowded, constantly fluctuating, and interaction-rich cellular environment.
