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We performed an assay in C. elegans based on the hypothesis that some of the binding partners of human LINKIN would also be conserved in C. elegans.
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Searching for possible immunocontraceptive targets in human spermatozoa by using yeast two-hybrid system, Naz and Dhandapani [19] recently identified the ubiquitin-binding, proteasome-associating protein UBAP2L as the most likely binding partner of human ZPC protein.
Similarly, for LR11/SorLA, a binding partner of the human amyloid precursor protein, a H N-TROSY spectrum recorded in isotropic bicelles was used as a 'gold standard' in screening for a suitable detergent system.
We used yeast two-hybrid analysis to identify potential protein binding partners for human TTP (hTTP).
During our efforts to isolate potential binding partners of key secretory proteins of M. tuberculosis from a human lung protein library, we isolated peptides that strongly bound the virulence determinant protein Esat6.
One of the key binding partners of β-catenin is Chibby (Cby), a small (126 residues in human) and highly conserved protein, which was first identified as an antagonist of Wnt signaling in 2003 (9).
To identify new binding partners of STK38, two independent yeast-two-hybrid (Y2H) screens were conducted with human full-length STK38 wild-type (WT) or constitutively active STK38-PIF [ 15] as baits.
The interference of recombinant soluble human IL-13 R α2 (one of the potential binding partners of IL13) was evaluated on the quantification of IL-13 at pH 5 and pH 3.5.
Thyropin is a domain found as a repeat in the amino terminal region of human thyroglobulin that is proposed to be an inhibitor of cysteine proteases and binding partners of heparin [ 73, 74].
In this study we performed a yeast two-hybrid screening using a human colon cDNA library and found that Grb2 and GrpL are binding partners of HD-PTP.
So far, it is unknown whether undiseased human brain endothelial cells or those in MS CNS lesions express the established binding partners of sVCAM-1, i.e. integrin α-4 heterodimers.
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