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Based on this general principle, the assay is designed to detect and identify protein binding partners of a protein complex.
Furthermore, using a peptide pulldown approach in combination with stable isotope labeling in cell culture (SILAC) we identified SLP-76, PLCγ, PIK3R1, Nck, CRK, Gads, and RasGAP as phospho-dependent binding partners of a central YDDV motif of ADAP.
While deviations of the predicted values from experimental individual single mutation results have been observed, FoldX performs well in identifying trends of effects of mutations and has been successfully used in several studies, in combination with homology modelling, to identify potential binding partners of a given protein [33], [39], [40].
All the binding partners of a biomolecule are listed on the 'Biomolecule Report' page.
Further, we propose that our in vivo overproduction system could be exploited as an efficient tool to identify the binding partners of a protein.
With affinity purification and mass spectrometry, binding partners of a protein can be interrogated in an untargeted fashion, offering important insight to protein function [ 75].
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The present structural results convey a simultaneous description of both binding partners of an LRP ligand complex and open a route to a broader understanding of the binding specificity of the LRP receptor, which may involve a general four-residue receptor ligand recognition motif common to all LRP ligands.
Identifying the binding partners of an unknown protein may shed light into its potential biological function.
The binding partners of an enzyme define its sites of action and regulatory mechanisms.
Bora was first identified as a binding partner of Aurora A in Drosophila (Hutterer et al. 2006).
One of the main binding partners of bacterial PDF is a trigger factor, a chaperone.
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