We also identified an additional 4 binding partners, including the single PDZ domain containing STN-2, a gamma syntrophin, and MPZ-1 that can be found at synapses.
Following activation by EGF-induced dimerization and autophosphorylation, EGFR recruits a group of binding partners including the scaffolding protein Grb2 to transmit proliferative and other signals.
ELF3 is known to have a number of binding partners, including the red-light photoreceptor PHYB, the ubiquitin E3-ligase COP1, and clock-related proteins GI, SVP, and CCA1, suggesting that ELF3 may function in large signaling complexes.
A second group of Ena/VASP binding partners, including the formin-family protein Diaphanous (Grosse et al., 2003; Schirenbeck et al., 2006; Barzik et al., 2014; Bilancia et al., 2014) and the WAVE regulatory complex (Law et al., 2013; Chen et al., 2014), relies on a related, but lower affinity, EVH1-binding motif: LPPPPP.
Mutation of Ser-286 to alanine or glutamic acid does not perturb the interaction of Gro/TLE1 with DNA-binding partners, including the basic helix-loop-helix transcription factor Hes1, a prototypical anti-neurogenic WRP(W/Y) motif protein.
In addition, since Arg contains multiple protein−protein interaction domains, Arg likely further facilitates N-WASp stimulation by putting it in the proximity of its other binding partners, including actin filaments, cortactin, and the Nck adaptor protein.
However, the 14-3-3 14-3-3 14-3-3believed to have several hundred direct binding proteins, including mare central regulators of the cell cycle, and their cellular localization may thus depend on other factors than Wee1 [ 27].
In a study on FER function using the Trojan peptide, researchers showed that FER that had dissociated from the cadherin complex could translocate to the integrin complex where it reduced p130CAS phosphorylation and interrupted integrin-mediated focal adhesion by affecting the phosphorylation of p130CAS binding partners, including PTP-PEST, PTP1B, FAK, and Crk [ 62].
Immunoprecipitation of NPM ALK from the Karpas 299 cell lines with monoclonal and polyclonal antibodies led to the identification of 36 NPM ALK binding partners, including both known and novel ALK-interacting proteins [ 137].
Exogenously expressed fusion proteins of NCS-1, containing a C-terminal GFP-variant moiety, correctly localise to the Golgi complex and plasma membrane in various cell types, and appear to colocalise at the Golgi with known NCS-1 binding partners including ARF-1 and PI4KIIIβ [50], [51], [52], [52].
PARylation of the protein alters its interaction with other binding partners, including proteins and nucleic acids.
