Contrary to our structure-based analysis, if two proteins share a binding partner in these high-throughput data, it does not necessarily mean that they are interacting simultaneously (Kim et al., 2006a).
Most proteins bind their binding partners in the native confirmation.
Cotelle, V. et al. 14-3-3s regulate global cleavage of their diverse binding partners in sugar-starved Arabidopsis cells.
These studies reveal mechanisms and antagonistic partners for specific IAPs, and provide a powerful technology for labeling binding partners in transient protein-protein complexes.
The molecular mechanisms underlying Golgi localization of isothiouronium-modified compounds were investigated using 7 CHO and 4 human cancer cell lines and the results indicated that the compounds had binding partners in the Golgi.
A micro-fluidic immobilisation device allowing the covalent attachment of up to 16 binding partners in a linear array on a single surface has been developed for compatibility with a prototype multiplex SPR analyser.
The fluorescence arm of the screen, however, would detect binding partners in pools containing pore blockers.
Moreover, the literature supports the role of 14.3.3 proteins in cleavage of their binding partners in sugar-starved cells [71].
A possible strategy for target identification is biotin-labeling, followed by affinity capture of binding partners in cell lysates.
Photounbinding was visualized and quantified by rebinding the same, but differently labeled binding partners in the previously illuminated (photobleached) areas.
