Sentence examples for binding partners from a from inspiring English sources
Y2H screening is an in vitro genetic approach to identifying binding partners from a library of polypeptides.
THL1 and THL2 were identified as SRK-binding partners from a yeast two-hybrid screen [ 49] and which act as negative regulators in SRK signaling [ 50, 51].
Biophysical and bacterial two-hybrid methods were used to initiate a search for potential binding partners from known components of the T7SS.
In vitro peptide binding studies suggest the BARD1 BRCT repeats may bind serine-phosphorylated peptides, although attempts to isolate phosphorylation-dependent protein binding partners from human cells for the BARD1 BRCT region have been unsuccessful.
For PDZ domains that recognize C-terminal sequences, the information has proven useful in identifying natural binding partners from genomic databases.
Since we had no clue about the identity of the potential GNE binding proteins in muscle cells, we applied the BIAcore system as a bioassay similar to the one described by Catimel et al. [24], who immobilized proteins onto the sensor chip and affinity purified binding partners from chromatographically enriched cell lysates.
This feature is particularly useful to isolate binding partners from complex mixtures such as cell lysates.
In addition also the purified NgR2 was analyzed, to ensure only binding partners from brain lysate are considered.
Recovery of CD44 and its binding partners from raft versus non-raft membrane microdomains was profiled in non-migrating and migrating breast cancer cell lines.
Oct4 has been proposed to be important for promoting mesendodermal differentiation (Thomson et al., 2011) and part of this mechanism might be explained by switching binding partners from Sox2 to Sox17 (Aksoy et al., 2013).
Furthermore, binding partners from multiple tissues and cell lines may need to be obtained, given certain nesprin variants have distinctive localisations and therefore probable altered scaffolding functions in different cell lines.
