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Alternately, the materials were malemided or gold-coated to provide bio-orthogonal binding partners for the thiol groups newly expressed on the cell surface.
We also performed a separate kinase assay, directly comparing the effect of binding partners for the dimerizing Arg117Gly and the non-dimerizing Arg95Ter.
Based on these initial findings, we screened a fluorescent small molecules based library in order to identify fluorescent binding partners for the DAPK catalytic domain, which could serve as reporters of the protein dimerization state.
Because of this extraordinarily high degree of variation in the ligand binding site, as well as relatively modest binding affinity (Kd of tens-to-hundreds µM), it is extremely difficult to predict binding partners for the large BRD family with knowledge of only three available structures of BRD and acetyl-lysine-peptide complexes [6].
Among them, 710 proteins were proposed to be binding partners for the Dvl PDZ domain [ 97].
Unfortunately, in the literature there are no identified binding partners for the SH3 domain of Caskin1.
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Although no definitive binding partner for Plxdc2 has been identified, several studies have isolated nidogen as a binding partner for the related protein, Plxdc1 [43], [43].
In Xenopus kermit2 was originally isolated as a binding partner for the IGF receptor [25] and has been implicated in regulating IGF signaling through interactions with the IGF receptor [26].
The symmetry of the top returned E-box motifs is suggestive of homodimer binding, consistent with the fact that C. elegans bodywall myogenesis occurs in the absence of the E protein binding partner for the MRFs typically found in other biological systems [17].
Based on quantitative membrane proteomics identification of novel membrane-associated proteins down-regulated by MARCH-VIII in primary human fibroblasts, we now show that the ER resident chaperone Bap31 is a binding partner for the majority of MARCH proteins and that Bap31 controls their intracellular transport.
CstF-50 is the best characterized binding partner for the BARD1 C-terminal region.
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binding energies for the
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