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DARPin libraries, based on a Designed Ankyrin Repeat Protein consensus framework, are a rich source of binding partners for a wide variety of proteins.
There is mounting evidence for a broad range of potential binding partners for a given START domain.
The Leu179Ala, Ile181Ala, and Val182Ala mutations were screened singly and in combinations using biolayer interferometry (BLI) with a modestly shorter gp120165 182 peptide to identify improved potential binding partners for a liganded CH58-UA structure (Fig. 2, Tables S1 S3).
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By screening of a peptide phage display library, we previously identified profilin-1 as a binding partner for a diabetic aorta-specific phage in rats [8].
In many cases, the development of these peptides has resulted from an understanding of the specific protein-binding partners for a particular protein kinase.
The extracellular matrix is rich in potential interaction and binding partners for periostin, a few of which have been elucidated in some detail.
To elucidate potential cellular binding partners for Tip-1, a LexA yeast two-hybrid screen was carried out using Tip-1 as bait.
However, the molecular bases for the functions of ADAMs are largely unappreciated, and identifying the in vivo substrates and binding partners for ADAMs remains a major challenge [ 2, 23].
Beyond this, and taking into consideration the current pace of advances in MS techniques, even the identification of whole SH2 interactomes representing a systematic analysis of all binding partners for SH2 domains in a specific organism may be lurking just behind the horizon.
A binding event between two proteins typically consists of a diffusional search of binding partners for one another, followed by a specific recognition of the compatible binding sites resulting in the formation of the complex.
Removing BMH2, in contrast, removes a smaller proportion of binding partners for Rad53, thus resulting in a milder suppression effect on cdc13-1.
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