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Each HP1 subunit consists of a chromodomain, which binds to methylated H3K9, a hinge implicated in the regulation of the protein/DNA/RNA interactions, and a chromoshadow domain, which is responsible for dimerization and for the interactions with binding partners containing the defined motifs comprise of the PXVXL consensus.
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SH2 domains bind to short amino acid sequences of potential upstream binding partners containing a tyrosine, whose phosphorylation enhances binding affinity.
The C-terminal domain of SSB is responsible for interacting with protein binding partners contains a short evolutionary conserved sequence motif (E. coli and B. subtilis PPMDFDDDIPF and PIDISDDDLPF, respectively).
Many of myosin VI's binding partners contain regions of predicted coiled coil and leucine zippers and there is preliminary evidence that one of them forms stable dimers [83].
The conservation of these residues implies the existence of an unknown NOXR binding partner(s) containing a PB1 domain with an OPCA motif.
We used statistical enrichment to identify protein signatures (domains, motifs) most likely to be found among binding partners of the proteins containing domain-altering SNPs.
The fluorescence arm of the screen, however, would detect binding partners in pools containing pore blockers.
The observation that the majority of cdh23's potential partners contain a calcium-binding domain is interesting since the intracellular domain of cdh23 is located where calcium concentration is highly regulated.
Most proteins bind their binding partners in the native confirmation.
We also identified an additional 4 binding partners, including the single PDZ domain containing STN-2, a gamma syntrophin, and MPZ-1 that can be found at synapses.
The presence of LMO4 in a complex containing the binding partners Ldb1, CtIP and the familial breast cancer tumour suppressor BRCA1 provides further compelling evidence for LMO4 playing a significant role in breast cancer pathogenesis [ 21], and activating mutations might be predicted to occur in some tumours and even in the germline of some patients.
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