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The ELM resource annotation did not include information about the binding partners and binding domain for each ELM class.
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Here we present the methods used to model the voltage-dependent gating of calcium channels and calcium influx, the binding of calcium to various intracellular binding partners, and the establishment of intracellular calcium homeostasis.
It provides information on binding sites, binding partners and descriptions of the relationships between the protein and its binding partners.
Determining the correct binding partners and predicting the ligand binding sites in the absence of experimental data require predictive models.
The outcome of the reuse of the binding pocket by multiple distinct binding partners and the required complementarity between binding peptide and the binding pocket results in the repeated patterns that we refer to as motifs.
Electrostatic interactions can facilitate macromolecular recognition by enhancing the collision frequency of the binding partners and by orienting the binding partners for subsequent short-range interactions.
This binding may allow the hijacking of host protein binding sites from their usual binding partners and thus alter the signalling pathways of the host cell.
We used GATHER [ 31, 32] to input the lists of 83 upregulated and 32 downregulated probe identifiers and generate annotations for gene ontology categories, KEGG pathways, protein binding partners and transcription factor binding sites.
Biochemical and structural analyses have contributed to detailed knowledge about potential binding partners and the understanding of how their binding may be regulated.
SPR is by now a well-established real-time, label-free means of robustly determining the binding constants and affinities of proteins for antibodies, ligands or other binding partners, and the binding of NTS1 to NT in detergent has already been demonstrated [3,32].
Consequently, it is possible that these tags will interfere with binding of actin binding partners and perturb actin function when transfected in cells, alternatively tags may interfere with the folding machinery.
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