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The level of SMC1, another core cohesin subunit and RAD21's binding partner, was not affected (Supplementary Figure 1A).
Closer analysis of the supernatant fractions indicated that, although the target protein was completely depleted, a reciprocal reduction in its coprecipitated binding partner was not observed.
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Thus the bulk of the evidence still suggests that a trGTPase binding partner is not required for termination or NGD in archaea.
They provide evidence that other binding partners are not expressed in the same area of the hippocampus.
As the binding partners are not attached to a label or surface, molecular properties are not altered and mobility is not restricted.
Another possibility to explain the multiple functions of RACK1 is that although the protein itself is ubiquitously expressed, its binding partners are not.
Her partner was not supportive.
Since the property of binding to a WT partner is not shared among the IDH mutants, it is unlikely that binding to a WT partner is an important feature of the IDH mutations in driving cancer pathogenesis.
In standard MST one binding partner is fluorescently labeled.
Although the relevant residues for binding to the sarcoglycan partners are not known, it is expected that some of the missense mutations residing in this region compromise the correct assembly of the complex.
The requirement of two independent motifs for the optimal binding of Vav proteins to other partners is not unprecedented.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com