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The addition of a lateral binding partner to all, or a subset of subunits along the filament, either through a neighbouring matrix or indeed another filament creates opportunities for complex and emerging properties of the resulting systems.
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YAP does not have DNA binding capabilities and requires a binding partner to activate gene transcription.
Through various interactions, molecular recognition occurs between protein and ligand, which is critical for identifying binding partners to the binding site.
Activated Smad2/3 forms complexes with Smad4, a DNA-binding partner common to all receptor-regulated Smads, and translocates into the nucleus.
Given that BUBR1 was isolated as a binding partner of all B56 subunits, we wished to determine if the B56 BUBR1 interaction is essential for promoting chromosome congression.
These binding partners all bind to two 'hot spots' either the RRL or WWY specific sites on the C-terminal cargo binding tail domain (Table 1) and may thus regulate the functions of myosin VI by helping to unfold and stabilise the tail and by recruiting the active molecule to specific intracellular locations [49].
Here we show BUBR1 as a direct binding partner of all B56 family members using yeast two-hybrid screening.
NHERF2 associates with all three ERM, but EBP50 appeared to be a weak binding partner if at all.
The unexpected presence of Kaiso at the mitotic spindle and the centrosomes prompted us to reexamine more precisely the expression of Kaiso's binding partner p120ctn at all stages of mitosis.
By tagging every protein with either a fluorescent protein or an affinity tag, they have made it possible to look at the localization, amount, and binding partners of all proteins.
Expression data is available for two of the three E2F transcription factors and both DP1 binding partners, and all are maximally expressed at 2 and 12 hours post-infection, though they are expressed at some level at all time points [ 25].
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