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We hypothesized that the mobile binding partner is the viral genome.
A key binding partner is the calcium-sensing protein CaM (calmodulin).
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One of actin's best-studied binding partners is the small ubiquitously expressed protein, profilin.
Candidates for these binding partners are the BH3-only proteins, which like BIM, have been shown to have higher affinities for anti-apoptotic BCL2 family members and can only bind weakly to BAX [ 30].
The oncogenic functions of E6 occur through its interaction with a number of cellular regulatory proteins and one of the best characterized E6-binding partners is the E6-associated protein (E6-AP) [ 7, 8].
While detection of this binding partner is beyond the scope of this work, future efforts will be directed toward the identification of the binding partner and elucidating its interaction interface.
Our use of the term "cooperativity" is based on previous studies, which have shown that the binding of RanGTP and the NES containing cargo to Crm1 is cooperative i.e. the affinity for each binding partner is increased in the presence of the other.
Furthermore, the observed effect that mutations at the periphery of the interface influence the binding affinity, even though the mutated residues are not directly contacting the binding partner, is in concordance with the theory that those residues affect the population equilibrium of the different conformers and thereby indirectly affect binding [25].
Of course, analyzing the various features of the binding partner is important for understanding the ligand-binding behavior.
The binding partner is also fairly constant throughout the day and so the complex concentrations can also be reasonably approximated as constant.
This hybridization capture strategy also prevents either binding partner from being subjected to harsh regeneration conditions, in contrast to systems in which one binding partner is fixed to the surface and may degrade over time.
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