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We previously defined Annexin 2 (asx2) an an HIV Gag binding partner in human monocyte-derived macrophages (MDMs) that promotes proper viral assembly.
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Experimental approaches for finding virus protein binding partners in the human proteome have proved challenging because nearly thirty thousand human proteins must be tested [ 7].
Based on the in vitro evolved sequence pattern we predict a large number of novel DYNLL binding partners in the human proteome.
Based on the phage-selected binding pattern we applied a simple scoring system combined with an untraditional baseline correction approach to identify novel natural binding partners in the human proteome.
Ska1 was originally found in a proteomic survey of the human mitotic spindle (Sauer et al, 2005), whereas Ska2 was identified as a Ska1 binding partner in a yeast two-hybrid screen (Hanisch et al, 2006).
However, Ku70 was not identified as a dysbindin-1 binding partner in our pull-down screening.
A candidate binding partner in the cytoplasm is PFK2/FBPase2 [11,12].
Both CysO and MoaD form a key binding interaction with their respective binding partner in this region.
Most proteins bind their binding partners in the native confirmation.
Vertebrate studies show neuroligins and neurexins are binding partners in a trans-synaptic cell adhesion complex, implicated in human autism and mental retardation disorders.
Alternatively, the binding partners in such species may be different from those in humans.
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