DARPin libraries, based on a Designed Ankyrin Repeat Protein consensus framework, are a rich source of binding partners for a wide variety of proteins.
There is mounting evidence for a broad range of potential binding partners for a given START domain.
The Leu179Ala, Ile181Ala, and Val182Ala mutations were screened singly and in combinations using biolayer interferometry (BLI) with a modestly shorter gp120165 182 peptide to identify improved potential binding partners for a liganded CH58-UA structure (Fig. 2, Tables S1 S3).
In addition, MED28 has been found to be a binding partner for merlin, a cytoskeleton-related tumor suppressor important in neurofibromatosis 2 development [ 12, 14].
In addition, because N. vectensis and other early diverging animals lack Period genes, we have no hypothesis for a binding partner for TIMEOUT in a potential repressor loop of the circadian clock.
In our previous study by a yeast two-hybrid screening [ 21], mEH was identified as a specific binding partner for HBSP from a human liver cDNA library.
Chunling Yi from Joseph Kissil's lab (The Wistar Institute) identified Angiomotin as a novel binding partner for Merlin, establishing a physical link between Merlin and the tight junction-associated Crumbs apical polarity complex [ 24].
By means of phage display technology, we selected a capable binding partner for one strand of a coiled coil bearing a charged amino acid in a central hydrophobic core position.
Of the three members of the Sir2/3/4 complex, only Sir4 has been identified as a binding partner for Ku by screening a two-hybrid library (Tsukamoto et al., 1997; Roy et al., 2004).
Thus, although we did not observe the up-regulation of erbB2 at this specific time point (50 days of age), an up-regulation of erbB3 could provide ample access to a binding partner for erbB2 and provide a local environment more prone for cancer development.
