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The kinetic binding parameters were calculated using the software of the manufacturer.
Binding parameters were calculated with the LIGAND program of Munson and Rodbard (1980).
Binding parameters were calculated using the Origin5 software using the 'One Set of Sites' curve fitting model provided by the software.
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By batch equilibrium binding analysis and dilution heat of peptide solution measurement, the binding isotherm and adsorption enthalpy were obtained and the binding thermodynamics parameters were calculated and analyzed.
The binding constant (Kd) and thermodynamic parameters were calculated from the average of three separate titrations by fitting the data to a single-site binding model using nonlinear least-squares fitting.
The kinetic parameters were calculated after background (PC/PE binding) subtraction.
The kinetic parameters were calculated after background subtraction (binding to PC/PE liposomes) exactly as described (Honing et al., 2005; Jackson et al., 2010; Kelly et al., 2008 ).
Thermodynamic parameters were calculated.
Various kinetic parameters were calculated.
The pharmacokinetic parameters were calculated using WINNONLIN.
The following parameters were calculated from the resulting linear function.
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