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The stability of the complex in the presence of ribonuclease (RNase) points either to direct interaction, which is unlikely because mutant pre-let-7a-1@3 binds Lin28a, but not Trim25, or close proximity binding on the same RNA.
Inhibition of bladder cell binding was performed simultaneously as binding on the same 12-well plate, but with the bacterial inoculum pre-incubated with different concentrations of mannose or HM.
Some, but not all, functions of CD22 are controlled by ligand binding on the same cell surface (cis) or via cell-cell interactions (trans) [ 136, 137].
The positioning was such that the operator was 10 base pairs downstream of the TATA box, resulting in repressor binding on the same side of the helix as the RNA polymerase.
In general the Hill equation is employed to describe the phenomenon that binding of a ligand to a macromolecule influencing the other ligands binding on the same macromolecule, which is known as cooperative binding.
Examples of this approach include the use of nucleoprotein filaments to mask a dsDNA scaffold locally from a subsequent metalization process and to create both a metal binding site and a region unsusceptible to metal binding on the same DNA molecule.
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In our previous experiments on HSA binding to the same type of nanoparticles we found that the binding data were well fitted by a simple binding model devoid of any apparent cooperativity or inter-protein interaction phenomena [1].
Therefore, we propose either that ToMOD and ToMOC compete for binding to the same location on ToMOH or that ToMOD enforces allosteric effects on ToMOH, inhibiting ET from ToMOCred to ToMOHox throughout turnover.
Hence, sequence changes that increase the origin efficiency of one ORC binding site may reduce selective pressure on ORC binding sites on the same chromosome (especially nearby), resulting in weaker binding sites or even sites with specialized function such as the silencers.
p50 binding to the same sites on these genes either did not change or increased, supporting the idea of p50 Bcl-3 binding complexes.
Thus, Wapl and α-kleisin might compete for binding to the same site on Smc3.
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