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Overall, these data suggest that a difference in the conformations of some side chains with rather less difference in backbone conformations is sufficient to accommodate the binding of two different phosphopeptides by the 14-3-3ζ 14-3-3ζ 14-3-3ζ
Numerous enhanced properties of polymer inorganic nanocomposites result from the mixing or the binding of two different phases.
Mad2 forms a conformational homodimer, as its dimerization requires the binding of two different stable conformations to each other: open, O-Mad2, and closed, C-Mad2 [ 46].
Furthermore, the hybridization kinetics of binary probes is slower than that of MB because BP hybridization depends on the binding of two different components to the target.
Subsequently, this maquette system was extended in a variety of ways to achieve coupling to electrode surfaces, incorporation of non-natural amino acid ligands, and binding of two different hemes which mimics the structure of ba3 oxidases.
Indeed, biochemical analysis supports the simultaneous binding of two different proteins to the homotrimeric human PCNA (Doré et al, 2009), and furthermore, biophysical analysis has demonstrated simultaneous binding of distinct proteins to the homodimeric Escherichia coli β clamp (Indiani et al, 2005) and the homotrimeric euryarchaeal PCNA (Kiyonari et al, 2009).
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Electrophoretic mobility-shift assays (EMSA) show that TNF-α decreased binding of three different complexes.
As a demonstration study, binding of three different biotinylated serum proteins (B-IgG, B-A, and B-TF) to the 2D panel was investigated.
Moreover, each mutant specifically led to the loss of binding of three different IDR-A or -B-specific rhFaffectingoutheffecting the binding of autoantibodies to the other determinant.
We observe a correlation between N-terminal binding of three different inhibitors (curcumin, resveratrol, and RSTWESKWR) and capping of the low MW oligomers.
Large-scale chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) studies have explored in vivo binding of five different STATs in a number of different cell types exposed to several cytokines.
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